BCI Pathology Services

Overview

Our Pathology Service offers a full range of histological techniques to all Barts Cancer Institute (BCI) centres.
The lab provides a service to the researchers at BCI who are working on projects in understanding the links between cancer and inflammation;
basic and applied PI3K signalling; cancer screening and prevention; therapeutic and diagnostic approaches to cancer;
and the roles played by cytoadhesion in modulating cancer spread.

 We also provide a service to other QMUL departments and to external users.

Key Services

• Frozen tissue samples - processing and cutting of sections.

• Microtomy: Section cutting from standard (FFPE or PFEF) or mega-blocks, on Palm/LCM membrane slides, standard or adhesive/positively charged slides at various thickness (usually from 3 to 10 microns, standard being at 4 microns)

• Tissue processing by automation and embedding into paraffin blocks:

1. Formalin fixed tissue (FFPE)
2. PAXgene tissue-fixed (PFPE)

• Automated Immunohistochemistry (IHC) singleplex or multiplexing staining of frozen or paraffin embedden sections.

• Tissue Microarray (TMA): TMA constructed by user (after training) or by the core as a service.

• Staining:

1. Haematoxylin & Eosin (H&E) staining of frozen and paraffin sections by automation.
2. Special stains:  Sirius Red, AB/PAS, Oil Red O, Congo Red, Masson Trichrome, Elastic Van Gieson (Miller), MSB, Retic, Grocott, Neutral red, MGG. 

• Visium Spatial slides:

1. section from standard FFPE (formalin fixed paraffin embedded), 10 microns,
2. section from frozen samples, 10 microns,

• RNAscope:

1. section cutting for hybridization
2. singleplex or multiplex, by automation, using Discovery ULTRA system

Location

Room 027 Ground Floor,
Wolfson Institute Building

Charterhouse Square,
London EC1M 6BQ  

Advice

As well as providing a high quality service, we offer advice on existing procedures.
We are also open to collaborations on developing new procedures and techniques, so please get in touch about any potential new projects.

Links and Resources

1. Visit our website here

Contacts